Robert Koch thus has established the relationship between a specific microorganism and a specific disease. The criteria for this can be generalized as: -
- The microorganism must be present in every case of the disease;
- The microorganism must be isolated from the diseased host and grown in pure culture;
- The specific disease must be reproduced when a pure culture of the microorganism and inoculated into a healthy susceptible host and
- The microorganism must be recoverable once again from the experimentally infected host.
These criteria has been generalized as Koch’s postulate to show respect to Koch’s work.
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